나노방출제어시스템을 이용하여 trichloroacetic acid와 epidermal growth factor의 순차적 방출을 적용한 인간치은섬유아세포의 세포생존 관련 유전자 연구분석
Analysis of cell survival genes in human gingival fibroblasts after sequential release of trichloroacetic acid and epidermal growth factor using the nano-controlled release system
조준연, 이성복, 이석원,
소속 상세정보
조준연 ( Cho Joon-Youn ) - Kyung Hee University School of Dentistry Institute of Oral Biology Kyung Hee University Hospital at Gangdong Department of Biomaterials & Prosthodontics
이성복 ( Lee Sung-Bok ) - Kyung Hee University School of Dentistry Kyung Hee University Hospital at Gangdong Department of Biomaterials & Prosthodontics
이석원 ( Lee Suk-Won ) - Kyung Hee University School of Dentistry Kyung Hee University Hospital at Gangdong Department of Biomaterials & Prosthodontics
Abstract
목적: 본 연구에서는 구강연조직재생에서 인간치은섬유아세포에 hydrophobically modified glycol chitosan (HGC) 기반 나노방출제어시스템을 적용 시 PI3K-AKT 신호전달의 세포생존 연관 유전자를 통해 trichloroacetic acid (TCA)와 epidermal growth factor (EGF)의 영향을 확인하고자 하였다.
연구 재료 및 방법: TCA와 EGF를 방출하는 나노방출제어시스템을 제작하였다. 실험군은 3가지 군으로 나누었다; 대조군(CON), TCA-담지형 나노방출제어시스템 투여군(EXP1), TCA-와 EGF- 담지형 나노방출제어시스템 투여군(EXP2). 인간치은섬유아세포 배양 후 PI3K-AKT 신호전달에 연관된 총 29개 유전자를 실시간 중합효소연쇄반응으로 분석했다. 일요인 분산분석 및 다중회귀분석이 사용되었다.
결과: 세포생존 관련 유전자들은 EXP1과 EXP2에서 상향조절되었다. 다중회귀분석에서는 ITGB1이 AKT1의 발현에 가장 영향력 있는 요소로 결정되었다.
결론: HGC기반 나노방출제어시스템을 통한 TCA와 EGF의 적용은 세포생존에 관한 신호전달을 상향 조절시킬 수 있다.
Purpose: This study was to determine the possible effects of trichloroacetic acid (TCA) and epidermal growth factor (EGF) through cell survival genes of the PI3K-AKT signaling pathway when applying an hydrophobically modified glycol chitosan (HGC)-based nano-controlled release system to human gingival fibroblasts in oral soft tissue regeneration. Materials and
Methods: An HGC-based nano-controlled release system was produced, followed by the loading of TCA and EGF. The
group was divided into control (CON), TCA-loaded nano-controlled release system (EXP1), and the TCA- and EGF- individually loaded nano-controlled release system (EXP2). A total for 29 genes related to the PI3K-AKT signaling pathway were analyzed after 48h of culture in human gingival fibroblasts. Real-time PCR, 1- way ANOVA and multiple regression analysis were performed.
Results: Cell survival genes were significantly upregulated in EXP1 and EXP2. From multiple regression analysis, ITGB1 was determined to be the most influential factor for AKT1 expression.
Conclusion: The application of TCA and EGF through the HGC-based nano-controlled release system can up-regulate the cell survival pathway.
키워드
인간치은섬유아세포; trichloroacetic acid; epidermal growth factor; PI3K-AKT 신호전달; 세포생존
human gingival fibroblast; trichloroacetic acid; epidermal growth factor; PI3K-AKT pathway; cell survival
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